Abstract: Bacteria of Escherichia coli harboring the expression plasmid, pBV220/PAI-1, might express recombinant PAI-1(rPAI-1) at the highest effeciency when the bacteria were induced at the bacterial density of A600nm= 0.3--0.4. Furthermore, the percentage of rPAI-1 in the bacteria kept rising within the 6 hours after the induction. The expressed rPAI-1 activated by guanidine HCl could form complexes with t-PA or u-PA, which might be visualized by SDS-PAGE or fibrin autography.

Key words: recombinant PAI-1, expression condition, inhibitory activity

摘要： 带有表达质粒pBV220/PAI-1的大肠杆菌中，在菌体密度为Ａ_600nm=0.3--0.4时诱导，表达重组PAI-1(rPAI-1)的效率最高。在诱导后的6h以内，rPAI-1的百分含量持续升高。rPAI-1经分离纯化并用盐酸胍激活后，能够与t-PA及u-PA反应，用SDS-PAGE和纤维蛋白自显影方法可以检测出它们所形成的复合物。

关键词: 重组PAI-1, 表达条件, 抑制活性