Abstract: Interleukin-6(IL-6) cDNA was obtained from the mRNA of human peripheral blood. Gene was cloned and expressed in E.coli. Sixty grams wet pellet was obtained from per liter fermentation liquid in 20L fermentation equipment. There were more than 30% IL-6 protein in the whole protein of the bacterium and 60% IL-6 protein in the inclusion body. Upon the basis of normal of long toxicity and short toxicity, the experiment on rhesus monkey shows that IL-6 can increase the quantity of platelet and leukocyte obviously without the model of decreasing hemogram.

Key words: interleukin-6(IL-6), expression of engineering bacterium, function

摘要： 自人外周血的mRNA中获得了白细胞介素6(Interleukin-6, IL-6)cDNA,基因被克隆并在大肠杆菌中表达，在20L发酵罐中每L发酵液可得60g湿菌体，IL-6的表达量约占大肠杆菌全蛋白的30%以上，占包含体蛋白的60%。纯化产物在长期，急性毒性和三致实验正常的基础上，用恒河猴的功能实验表明，在不制造血象降低模型的情况下，仍能明显增加血液中血小板和白细胞的数量。

关键词: 人白细胞介素6, 工程菌表达, 功能