Abstract: A phage display 15mer random peptide library expressed on the N-terminal of the filamentous phage M13 minor coat protein pⅢ was constructed using the phagmid pCANTAB5G8. The capacity of library is 1.36×10⁸, calculated from the transformation efficiency. Human recombinant Intercellular Adhesion Molecule 1 was immobilized on the ELISA plates. After 4 rounds affinity panning and ELISA analysis, 5 positive phage clones that can bind to ICAM-1 specifically were selected. These positive clones were sequenced and the amino acid sequence of 15mer were deduced.The Ka of one positive clone was 7.87×10⁷, measured by ELISA.

Key words: phage display, random peptide library, ICAM-1, biopanning

摘要： 利用噬菌体展示载体pCANTAB5G8构件了随机十五肽库，根据转化率计算库容量为1.36×10⁸。用PCR-SSCP检验了库的异质性。以固相包被亲和淘选的方法，经过4轮淘选和ELISA分析，得到了5个可与重组人细胞间粘附分子1（ICAM-1）特异结合的噬菌体克隆，对所展示的十五肽进行了序列分析。其中活性较高的噬菌体展示十五肽与ICAM-1的亲和常数约为7.87×10⁷。

关键词: 噬菌体展示, 随机多肽库, 细胞间粘附分子1, 亲和淘选