Abstract: Estriol (E₃) was bound to bovine serum albumin (BSA) to form a complete antigen (E₃-BSA), after the hydroxy (-OH) at the position of C3 was activated. A BALB/c mouse was immunized with the mixture of E₃-BSA and Freund adjuvant. Monoclonal antibody-producing hybridoma anti-E₃ was prepared by fusion of SP2/0 murine myeloma cells with spleen cell isolated from the immunized mouse. A monoclonal antibody (McAb) specific for E₃ was produced in vivo by the hybridoma anti-E₃. Analysis revealed that anti-E₃ McAb (E₃-McAb) was of the IgG1 type,with the molecular weight 168000 u. The titer of E₃-McAb was 6.0×10^-10 mol/L, and the affinity constant of E₃-McAb with coated complete antigen was 4.7×10⁷ L/mol.

Key words: estriol, cell fusion, monoclonal antibody

摘要： 活化雌三醇(E₃)C3处的酚—OH，与牛血清白蛋白(BSA)偶联，制备得E₃的完全抗原。利用完全抗原免疫动物，采用单克隆抗体技术，经过细胞融合、克隆筛选、扩大培养、动物体内诱生等过程，最后获得了E₃的单克隆抗体。对纯化的单克隆抗体的性质进行表征，结果表明：抗体的效价为6.0×10^-10 mol/L，抗体属于IgG1型，分子量为168000 u，单克隆抗体与固定化抗原亲和常数为4.7×10⁷ L/mol。

关键词: 细胞融合, 单克隆抗体, 雌三醇