Abstract: A simulated cultivation was carried out in lab, using the sediment and seawater samples collected from the discharge-receiving sea near an industrial park in Hangzhou Bay. The samples were collected from two sites-one was near to the outlet (site A) and the other was far from the outlet (site B). Ammonia oxidation was stimulated and enhanced by the addition of ammonium in the sediment-seawater microsystems. After 35-day cultivation, all of the external NH₄⁺-N was removed, but NO₃^--N accumulated in the aqueous solution. The PCR-DGGE detection, based on the ammonia monooxygenase subunit A (amoA) gene, showed that ammonia oxidation was coupled with bacterial instead of archaeal pattern. Under the shock load of ammonium, the numbers of Bact-amoA bands increased from 1 to 7 (site A) and 5 (site B), indicating that the abundance of ammonia oxidation bacteria (AOB) increased. However, the Arch-amoA bands stayed the same, which showed that the community structure of ammonia oxidation archaea (AOA) kept unchanged.

Key words: ammonia oxidation bacteria (AOB), ammonia oxidation archaea (AOA), ammonia oxidation, external ammonium

摘要： 采集杭州湾邻近工业园区纳污水域靠近排污点(A点)及远离排污点(B点)的沉积物和水样, 进行实验室模拟培养实验。在外源氨氮的刺激下, 培养体系中微生物氨氧化作用加强, 35天后水中投加的NH₄⁺-N全部被去除, 而NO₃^--N出现积累。源于A, B点的样品在培养实验后, 基于细菌氨单加氧酶基因(Bact-amoA)的DGGE指纹图谱中的条带数由1条分别增加至7条和5条, 表明氨氧化细菌的群落结构发生变化, 多样性增加; 而基于古菌氨单加氧酶基因(Arch-amoA)的DGGE条带数没有明显变化, 表明氨氧化古菌的群落结构没有显著变化。

关键词: 氨氧化细菌, 氨氧化古菌, 氨氧化, 外源氨氮