Abstract: The polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) was used to analyze the stability of microbial community structure in continuous white rot Fungal Membrane Bio-Reactor (FMBR) and the results were interpreted using sophisticated multidimensional scaling (MDS) analysis. The experiment was carried out for 100 days continuously. The results indicate that the Phanerochaete chrysosporium (P. chrysosporium) was identified by sequencing and it was the predominant species in microbial community. Then with the time increasing, the predominant was more and more obvious. The Shannon-Wiener indices of the microbial community at the end of the experiment decreased to 0. 92 lower than the average of 1. 92. The MDS analysis show that the changes among the microbial communities was relatively stable during the continuous FMBR operation stable. The average rate of decoloration maintained at 82.5% and the maximum rate could reach to 93. 2% . The continuous FMBR could decolorize dyestuff wasterwater efficiently during the operation.

Key words: white rot fungal membrane bio-reactor (FMBR), polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE), microbial community structures, multidimensional scaling (MDS), decoloration rate

摘要： 通过PCR-DGGE( 变性浓度梯度凝胶电泳) 分析评价了连续运行100 天的白腐真菌膜生物反应器中群落结构的变化, 并研究了群落结构与染料脱色效率之间的耦联关系, 且通过多维尺度(MDS) 方法对 DGGE 电泳图谱进行分析, 以二维图形向量的方式显示。结果表明, 通过对系统内优势菌种的 18S rDNA 序列进行测定, 并通过NCBI(美国国立生物技术信息中心) 基因库比对确定的菌种 Phanerochaete chrysosporium ( P. chrysosporium) 在运行期间始终是微生物群落的优势种, 随着系统运行时间的延长, 其优势愈加 明显, 但反应器 中微生物群落结构 Shannon-Wiener 多样性指数却逐渐降低, 由起始阶段的 1. 92 降至最终的 0. 92 。对 DGGE 条带进行 MDS 分析得知, 反应器在运行阶段基本稳定维持处在一个区域, 表明其微生物群落结构在运行期间较为稳定, 且该反应器对染料废水始终具有良好的脱色效果, 平均脱色率为82.5% , 最高时可达93.2%。

关键词: 白腐真菌膜生物反应器, PCR-DGGE, 群落结构, MDS分析, 脱色率