Abstract: Gene difference expression of the lung tissues between of mice and Microtus fortis infected with Schistosoma japonicum for 7d, and the lung tissues and the liver tissues of Microtus fortis infected with Schistosoma japonicum for 12d were analyzed by cDNA microarrays. The results showed that serine protease inhibitor genes expression was up-regulated and immune-associated genes expression were not obviously changed in the lung tissues of mice. On the contrary, serine protease inhibitor genes expressed constantly in the lung tissues of Microtus fortis infected with Schistosoma japonicum for 7d and Several non specific immune associated genes (such as lysozyme gene and cathepsin genes) were up-regulated. At the same time, some important specific immune associated genes(such as CD74、MHCⅡ and MHCⅠ) were up-regulated. The lung tissues and liver tissues of Microtus fortis infected with Schistosoma japonicum for 12d also confirmed the results by enhancing expression of the non specific immune associated genes and specific immune associated genes. Furthermore, in the lung tissues of Microtus fortis infected with Schistosoma japonicum for 12d serine protease inhibitor genes expression were down-regulated. It was suggested that the mice and Microtus fortis share reverse expression patterns, which was helpful to deeply understand the molecular mechanism of natural resistance of Microtus fortis to Schistosoma japonicum.

Key words: Microtus fortis, cDNA microarray, serine protease inhibitor, lysozyme, cathepsin, CD74, MHC Ⅱ, MHCⅠ

摘要： 利用大、小鼠基因表达谱芯片分别研究了Balb/c小鼠感染日本血吸虫7d时肺组织、东方田鼠感染日本血吸虫7d时肺组织、12d时的肺和肝组织基因差异表达方式。结果表明，小鼠感染日本血吸虫7d时肺丝氨酸蛋白酶抑制剂（Serine protease inhibitor）基因表达上调，同时没有免疫相关基因的表达变化；与之相反，东方田鼠感染日本血吸虫7d时肺丝氨酸蛋白酶抑制剂基因没有表达变化，非特异性免疫相关基因，如溶菌酶(Lysozyme)和各类组织蛋白酶(Cathepsin)基因表达上调，而且肺内特异性免疫相关基因如CD74、MHCⅡ和MHCⅠ等表达上调。这一现象得到东方田鼠感染日本血吸虫12d时肺和肝中特异性免疫和非特异性免疫相关基因的上调表达的进一步证实。而且，东方田鼠感染日本血吸虫12d时肺中丝氨酸蛋白酶抑制剂基因表达下调，肝组织中胰岛素样生长因子-1（Insulin-like growth factor 1，IGF 1）基因表达下调。提示日本血吸虫适宜性宿主小鼠和非适宜性宿主东方田鼠感染日本血吸虫前后两者有相反的基因表达方式。东方田鼠抗日本血吸虫机制可能包括免疫防御机制和阻止虫体获得宿主源促生长发育物质机制。

关键词: 东方田鼠, 基因芯片, 丝氨酸蛋白酶抑制剂, 溶菌酶, 组织蛋白酶, CD74, MHCⅡ, MHCⅠ