Identification and Expression of the Product from Engineering Bacterium Containing Recombinant Human Interleukin-18(IL-18) Gene

Acta Scientiarum Naturalium Universitatis Pekinensis

Previous Articles Next Articles

Identification and Expression of the Product from Engineering Bacterium Containing Recombinant Human Interleukin-18(IL-18) Gene

DONG Yuqing, LI Ronggui, DONG Chunxia, ZHAO Jindong, WU Guangyao

College of Life Sciences, Peking University, Beijing, 100871

Received:1999-03-04 Online:2000-03-20 Published:2000-03-20

含有重组人白细胞介素18基因的工程菌的产物表达和鉴定

董宇清, 李荣贵, 董春霞, 赵进东, 吴光耀

北京大学生命科学学院，北京，100871

Abstract

Abstract: The cDNA of human Interleukin-18 was obtained by RT-PCR from the mRNA of human peripheral blood cells. The DNA sequencing was performed and the DNA was cloned into expression vector pBV220. The overexpression was performed in E.coli HB101. The expressive product was purified through Sephadex G100 and identified by N-terminal amino acid sequencing and Western blot.

Key words: human interleukin-18 (hIL-18), gene cloning, expression

关键词: 人白细胞介素18, 基因克隆, 表达

CLC Number:

DONG Yuqing,LI Ronggui,DONG Chunxia,ZHAO Jindong,WU Guangyao. Identification and Expression of the Product from Engineering Bacterium Containing Recombinant Human Interleukin-18(IL-18) Gene[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

董宇清, 李荣贵, 董春霞, 赵进东, 吴光耀. 含有重组人白细胞介素18基因的工程菌的产物表达和鉴定[J]. 北京大学学报（自然科学版）.

[1]	ZHOU Bohan, CAO Jian, WANG Yuan. A Transformer-based Syntax Tree Decoder for Handwritten Mathematical Expression Recognition [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2023, 59(6): 909-914.
[2]	ZHANG Yu, ZENG Li, ZOU Lei. Regular Path Queries on Large Graph Data [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2018, 54(2): 236-242.
[3]	YANG Jinshui, GAO Quanxiu, LI Zhaosheng, XING Guanlan, YUAN Hongli. Clone, Expression and Function Analysis of Diacylglycerol Acyltransferase Gene from Chlorella variabilis NC64A [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2016, 52(2): 187-192.
[4]	WANG Wei,ZHAO Dongyan,SU Tingting. C-TERN: A Temporal Information Processing Algorithm of Chinese Military News Story Based on Cascade Finite State Automata [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2014, 50(1): 9-16.
[5]	WEI Jianping. Wave Propagationin Elastic Square Columns Ⅳ. Effect of Boundary Condition [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2009, 45(5): 743-747.
[6]	CHIEN Shu. Role of Stress Direction on Mechanotransduction and Functions of Endothelial Cells [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2007, 43(4): 435-440.
[7]	QIN Yan,CUI Honglian,HU Meihao. In Vitro Construction of Pseudovirions of Human Papillomavirus Type 16 [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2006, 42(2): 180-183.
[8]	ZHAO Jingjing,RU Binggen. Expression of Haemadin in Methylotrophic Yeast Pichia Pastoris and Its Purification and Characterization [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2005, 41(4): 506-513.
[9]	LI Min,WANG Yujiong,CUI Jingrong,HU Rongliang,ZHANG Shoufeng. Construction and Identification of t-PA Variants Gene Transfected Cell Lines [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2004, 40(4): 583-587.
[10]	LI Junguo,ZHANG Li'ang. A XML Based Presentation Technique for Mathematical Expressions on Web Page [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2003, 39(5): 675-680.
[11]	YU Qiuju,WU Qi,LIN Zhongping,LI Jingfu. Advance of Plant Aquaporins Research [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2002, 38(6): 855-866.
[12]	ZHOU Jianjun,QU Hong,WU Caihong,HAN Weidong,LI Zhiwei,FENG Meifu. cDNA Cloning, Sequence Analysis, Expression and Protein Structure Modeling of HLA-G1 from Chinese [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2002, 38(2): 212-220.
[13]	JU Wujian,HU Meihao. Introduction of A Linking Peptide to the Thrombolysis-Targeted Protein and Its Expression in Insect Cell [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2001, 37(6): 785-790.
[14]	WANG Hansong,YU Meimin,RU Binggen. Expression and Properties of the α Domain of Human Metallothionein-3 [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2001, 37(4): 470-476.
[15]	KOU Ruqin,WANG Wei,LI Lingyuan,RU Binggen. Clone and Enzyme-Linked Immunosorbent Assay of Human Intestinal Trefoil Factor [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2000, 36(6): 777-782.

Identification and Expression of the Product from Engineering Bacterium Containing Recombinant Human Interleukin-18(IL-18) Gene

含有重组人白细胞介素18基因的工程菌的产物表达和鉴定

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics