Cloning and Expression of the Rabbit Growth Hormone cDNA

Abstract

Abstract: The cDNA of preprotein and mature protein of rabbit hypophysiscerebri was obtained. The cDNA of mature growth hormone was cloned in pET-3a, then the recombinant expression vector was transformed into E.coli BL21(DE3 plys). The overexpression was performed and the yield was 40% of the total protein.

Key words: rabbit growth hormone, cDNA clone, expression

摘要： 从兔脑下垂体中的mRNA中，利用合成引物逆转录PCR，获得了前体和成熟生长激素的cDNA，成熟生长激素的cDNA克隆在pET-3a表达载体并转化到大肠杆菌BL21(DE3 plys)中，获得了高效表达，表达量达到大肠杆菌总蛋白的40%。

关键词: 兔生长激素, cDNA克隆, 表达

CLC Number:

Q344⁺.13

DONG Yuqing,LI Ronggui,ZHAO Jindong,WU Guangyao. Cloning and Expression of the Rabbit Growth Hormone cDNA[J]. Acta Scientiarum Naturalium Universitatis Pekinensis.

董宇清, 李荣贵, 赵进东, 吴光耀. 兔生长激素cDNA克隆和表达[J]. 北京大学学报（自然科学版）.

[1]	ZHOU Bohan, CAO Jian, WANG Yuan. A Transformer-based Syntax Tree Decoder for Handwritten Mathematical Expression Recognition [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2023, 59(6): 909-914.
[2]	ZHANG Yu, ZENG Li, ZOU Lei. Regular Path Queries on Large Graph Data [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2018, 54(2): 236-242.
[3]	YANG Jinshui, GAO Quanxiu, LI Zhaosheng, XING Guanlan, YUAN Hongli. Clone, Expression and Function Analysis of Diacylglycerol Acyltransferase Gene from Chlorella variabilis NC64A [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2016, 52(2): 187-192.
[4]	WANG Wei,ZHAO Dongyan,SU Tingting. C-TERN: A Temporal Information Processing Algorithm of Chinese Military News Story Based on Cascade Finite State Automata [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2014, 50(1): 9-16.
[5]	WEI Jianping. Wave Propagationin Elastic Square Columns Ⅳ. Effect of Boundary Condition [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2009, 45(5): 743-747.
[6]	CHIEN Shu. Role of Stress Direction on Mechanotransduction and Functions of Endothelial Cells [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2007, 43(4): 435-440.
[7]	QIN Yan,CUI Honglian,HU Meihao. In Vitro Construction of Pseudovirions of Human Papillomavirus Type 16 [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2006, 42(2): 180-183.
[8]	ZHAO Jingjing,RU Binggen. Expression of Haemadin in Methylotrophic Yeast Pichia Pastoris and Its Purification and Characterization [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2005, 41(4): 506-513.
[9]	LI Min,WANG Yujiong,CUI Jingrong,HU Rongliang,ZHANG Shoufeng. Construction and Identification of t-PA Variants Gene Transfected Cell Lines [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2004, 40(4): 583-587.
[10]	LI Junguo,ZHANG Li'ang. A XML Based Presentation Technique for Mathematical Expressions on Web Page [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2003, 39(5): 675-680.
[11]	YU Qiuju,WU Qi,LIN Zhongping,LI Jingfu. Advance of Plant Aquaporins Research [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2002, 38(6): 855-866.
[12]	ZHOU Jianjun,QU Hong,WU Caihong,HAN Weidong,LI Zhiwei,FENG Meifu. cDNA Cloning, Sequence Analysis, Expression and Protein Structure Modeling of HLA-G1 from Chinese [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2002, 38(2): 212-220.
[13]	JU Wujian,HU Meihao. Introduction of A Linking Peptide to the Thrombolysis-Targeted Protein and Its Expression in Insect Cell [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2001, 37(6): 785-790.
[14]	WANG Hansong,YU Meimin,RU Binggen. Expression and Properties of the α Domain of Human Metallothionein-3 [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2001, 37(4): 470-476.
[15]	KOU Ruqin,WANG Wei,LI Lingyuan,RU Binggen. Clone and Enzyme-Linked Immunosorbent Assay of Human Intestinal Trefoil Factor [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2000, 36(6): 777-782.

Cloning and Expression of the Rabbit Growth Hormone cDNA

兔生长激素cDNA克隆和表达

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics