Acta Scientiarum Naturalium Universitatis Pekinensis

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Like-Intermediate Filament System in Euglena gracilis

FENG Lei, GAO Chongming, LI Yinzhen   

  1. College of Life Sciences, Peking University, Beijing, 100871
  • Received:1998-12-21 Online:1999-07-20 Published:1999-07-20



  1. 北京大学生命科学学院,北京,100871

Abstract: After using three consecutive extractions and DGD-free process to treat Euglena gracilis, we get well-defined cytoskeleton network. The photos of electron microscopy show that like-intermediate filaments, which often appear short and in bundle, exist both in nuclear and in protoplasm. The diameter of a single filament is 12-14nm. The molecular weight determined by Western-blotting proves the main like-IF is a 66kD protein. The immunofluorescence illustrates that the 66kD protein mainly appears at the surfaces of organelle and plasma membrane.

Key words: Euglena, intermediate filament

摘要: 用分级抽提和DGD包埋去包埋技术处理小眼虫(Euglena gracilis),获得了完整的、清晰的细胞纤维网架结构。电镜结果显示纤维的直径在12~14nm,有时呈束状结构,通常以短纤维网络形式存在。免疫印迹证明类中间纤维蛋白的主要成分是一种66kD蛋白。免疫荧光显示类中间纤维蛋白主要分布在各类细胞器的表面,以及细胞表皮的下层。

关键词: 眼虫, 中间纤维

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