Acta Scientiarum Naturalium Universitatis Pekinensis

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Labeling Biotin with Tc-99m via N, N'-Biscysteinyl-Lysine

DONG Yanbing, WU Yonghui, LIU Bin, LIU Yuanfang   

  1. Department of Technical Physics, Peking University, Beijing, 100871
  • Received:1993-08-26 Online:1995-03-20 Published:1995-03-20

通过二(半胱氨酰)赖氨酸用99mTc标记生物素

董燕兵,吴永慧,刘斌,刘元方   

  1. 北京大学技术物理系,北京,100871

Abstract: A new biotin derivative was systhesized by conjugatingN, N'-biscysteinyl-lysine(BCL) to biocytin. The conditions of conjugationwas studied BCL-biocytine was labeled with Tc-99m. The labels was identified by TLC, GC and bonding with avidin. The biodistribution of the labels in normal mice was also investigated. The stability of Tc-99m-BCL-biocytine both in vitro and in vivo suggested that the labels may be further used in pretargeting.

Key words: immunoimaging, pretargeting, N, N'-biscysteinyl-lysine, avidin-biotin

摘要: 生物素(biotin)和亲和素(avidin)具有很强的亲和力,这一特性可以被用于改进放免显像的预定位技术。本文阐述了利用一种新的含硫含氮配体L-N-N′-二(半胱氨酰)赖氨酸(BCL)作为联接剂,用99mTc标记生物素的方法。初步研究的结果表明,标记物具有较好的体内外稳定性,可以进一步用于抗肿瘤单抗预定位,再注射能与亲和素结合的放标生物素进行肿瘤显像。

关键词: 放免显像, 预定位技术, 二(半胱氨酰)赖氨酸, 生物素-亲和素

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