By investigating 80 samples of ripe, unripe and processed rape honey collected from three regions (Anhui, Inner Mongolia and Jiangsu) in China, a method based on NOESY 1D combined with O2PLS-DA was developed to discriminate the mentioned three types honey. NOESY 1D spectra of representative honey samples were first compared, and some obvious differences among the three types of honey were observed. O2PLS-DA model was applied to discriminate the collected samples successfully. The segmental bin was selected based on the greatest R²Y and Q² values. Three types of honey from three regions could be successfully discriminated, although there was partially overlap among some of the unripe and processed honey samples, but they can be discriminated in 3D graph. The developed method could be used to identify ripe, unripe and processed honey from same region, all R²Y and Q² are higher than 0.97 and 0.81, respectively. This method could be applied to discriminate the ripe, unripe and processed rape honey from the same region. The advantages of this method are small sample amount, short time, good reproducibility and reliable results.

ripe honey, unripe honey, processed honey (concentrated honey), nuclear magnetic resonance Spectroscopy (NMR), orthogonal bidirectional projections to latent structures-discriminant analysis (O2PLS-DA)

应用核磁共振波谱法, 对采集自安徽、内蒙古和江苏3个地区油菜蜜的成熟蜜、非成熟蜜及加工蜜共计80个样品的氢谱进行测定, 建立区分3种蜂蜜的方法, 解析油菜蜜组分中未重叠的特征峰, 对比3个地区3种蜜代表性样品的核磁波谱图(NOESY 1D), 并分析不同蜂蜜中部分组分的含量。运用隐结构双向正交投影?判别分析法(O2PLS-DA), 对上述蜂蜜进行分组判别, 选取R²Y和Q²值最大的积分间隔, 可清晰地区分3个地区的成熟蜜。虽然加工蜜与非成熟蜜有部分重叠, 但在三维图中可以区分。对同一地区的3种蜂蜜可以清晰地分组区分, 3组R²Y值均大于 0.97, Q²值均大于 0.81。所建立的方法可用于鉴别同一地区的成熟、非成熟与加工油菜蜜, 具有样品用量少、检测用时短和结果重现性好等优势。