Six treatments (control (CK), litter removal (?L), litter addition (+L), root removal (?R), litter and root removal (?R?L), litter addition and root removal (?R+L)) were conducted in a Pinus sylvestris var. mongolica plantation in Saihanba, Hebei Province. Based on the results of the first growing season (May–September 2019) after the treatments, we aimed to evaluate the effects of different plant detritus input on soil respiration and its temperature sensitivity. The results show that: 1) soil respiration rate exhibited significant seasonal dynamics, with peak value in July–August and minimum value in late September. The average soil respiration rates of control, litter removal, litter addition, root removal, litter and root removal, litter addition and root removal treatments were (mean±SE) 2.20±0.17, 1.24±0.09, 2.40±0.07, 2.05±0.11, 1.04±0.07 and 2.28±0.17 μmol/(m2·s) respectively. Compared to the CK, ?L and ?R?L significantly reduced soil respiration rate by 43.6% and 52.7%, respectively; 2) soil respiration in the growing season was significantly and exponentially correlated with soil temperature (p<0.01) among different treatments. The temperature sensitivity of soil respiration (Q10 values) were 2.32±0.13, 2.37±0.05, 2.41±0.09, 2.42±0.14, 2.09±0.08 and 2.87±0.11, respectively. Aboveground litter treatment had significant effects on both average rate and Q10 value of soil respiration (p<0.05), while belowground root treatment did not (p>0.05); 3) compared to CK, soil moisture was significantly higher in all root removal treatments (?R, ?R?L, ?R+L) likely due to the lack of water uptake by roots, but soil temperature did not change significantly. In most treatments (except ?R and ?R+L), soil respiration and soil moisture showed a significant parabolic relationship during the growing season, and the threshold of soil moisture was about 15% (v/v). In conclusion, the results showed that litter removal had significant effects on growing season soil respiration and its temperature sensitivity in the Pinus sylvestris var. mongolica plantation, but root removal had no significant effect in the first growing season after the manipulation.