Acta Scientiarum Naturalium Universitatis Pekinensis ›› 2017, Vol. 53 ›› Issue (6): 1115-1121.DOI: 10.13209/j.0479-8023.2017.122

• Orginal Article • Previous Articles     Next Articles

Screening of Alginate Degrading Bacteria and Optimization of Its Enzyme-Producing Conditions

Tingting LI1,2, Qian CHEN2, Ping SHI1, Shu GENG3()   

  1. 1. Shenzhen Key Laboratory for Heavy Metal Pollution Control and Reutilization, School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055
    2. Beijing Engineering Research Center for Advanced Wastewater Treatment, Department of Environmental Engineering , Peking University, Beijing 100871
    3. Sino-US Joint Food Safety Research Center, Northwest A&F University, Yangling 712100;
  • Received:2016-05-12 Revised:2016-05-31 Online:2017-10-24 Published:2017-11-20

高效褐藻胶降解菌的筛选与产酶条件优化

李婷婷1,2, 陈倩2, 石萍1, 耿旭3()   

  1. 1. 深圳市重金属污染控制与资源化重点实验室, 北京大学深圳研究生院环境与能源学院, 深圳 518055
    2. 北京市新型污水深度处理工程技术研究中心, 北京大学环境工程系, 北京 100871
    3. 中美食品安全联合研究中心, 西北农林科技大学, 杨凌 712100
  • 基金资助:
    北京市科技专项(Z161100005016034)资助

Abstract:

53#, a strain of efficient alginate degrading bacteria, is isolated from rotted kelp using sodium alginate as the only carbon resource. Strain 53# is identified as Paenibacillus agaridevorans based on physiological characteristics and 16S rRNA sequencing. The optimaltion yield condition of strain 53# is identified at pH=8, T=25ºC, NaCl 15 g/L, and sodium alginate 15 g/L by orthogonal experiment and analysis, and the highest enzyme activity is 390.53±17.32 U/mL. Strain 53# has the advantages such as being cultivated easily, producing enzyme fast and having high enzyme activity. It can achieve a high efficiency for saccharification of alginate and thus has potential value to be utilized in the production of bioethanol from brown algae.

Key words: alginate degradation, alginate lyase, Paenibacillus, biofuel

摘要:

以海藻酸钠为唯一碳源, 从天然腐烂海带中筛选得到一株高效褐藻胶降解菌株53#, 经形态学观察和16S rRNA鉴定, 确定为类芽孢杆菌Paenibacillus agaridevorans。采用正交试验方法, 以pH、温度、NaCl浓度和海藻酸钠初始浓度为影响因素, 对该菌株的产酶条件进行优化, 得到53#菌的最佳产酶条件: pH=8, 25℃, NaCl浓度15 g/L, 海藻酸钠初始浓度15 g/L。在最佳产酶条件下, 褐藻胶裂解酶最大酶活可达390.53±17.32 U/mL。筛选得到的类芽孢杆菌Paenibacillus agaridevorans 53#具有易于培养、产酶速度快和酶活力高等优点, 能够实现褐藻胶的高效糖化, 在褐藻生产生物乙醇领域具有潜在利用价值。

关键词: 褐藻胶降解, 褐藻胶裂解酶, 类芽孢杆菌, 生物乙醇