Abstract: Polyclonal antibody to butachlor was prepared from rabbits immunized with butachlor-BSA conjugate, and a competitive ELISA was developed for the detection of the butachlor (a major component in a herbicide) within the range of 1×10^-9～100×10^-9mg/mL without cross reactions with the structurally similar chloroacetanilide herbicides, alachlor and acetochlor. This method could also be used to monitor the butachlor concentration of water samples from rice field.

Key words: polyclonal antibody, butachlor, ELISA

摘要： 以牛血清白蛋白-丁草胺免疫抗原免疫家兔获得了抗丁草胺多克隆抗体，并建立了竞争酶联免疫吸附检测丁草胺(除草剂中的一种主要成分)分析方法。该方法的检测范围在1×10^-9～100×10^-9mg/mL之间，且和化学结构相似的甲草胺和乙草胺无交叉反应。该方法也可用于检测稻田水样中丁草胺的含量。

关键词: 多克隆抗体, 丁草胺, 酶联免疫吸附分析