Acta Scientiarum Naturalium Universitatis Pekinensis

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A Preliminary Investigation on the Lamin-like Proteins in Paramecium

CHEN Qiushi,GAO Chongming   

  1. College of Life Science, Peking University, Beijing, 100871
  • Received:2001-02-22 Online:2002-03-20 Published:2002-03-20

草履虫类核纤层蛋白的初步研究

陈秋实,高崇明   

  1. 北京大学生命科学学院,北京,100871

Abstract: Paramecium caudatumwas first processed by DGD embedment and embedment-free technique and general technique for EM morphology. A perinuclear structure consisted of interacted filaments (we called lamina-like structure) was observed. Then using Western Blot assay, we found a lamin-like component band of 68kD protein in the three-step-fractionated cells. To investigate the distribution of the lamin-like protein in cells, a immunofluorescence experiment for in situ hybridization was designed using goat anti-lamin protein antibodies as the probes. The results revealed that the positive reactivity presented at different part of the cells. The perinuclear cross-actions were distinct, and cross-action with the oral apparatus and the cortex were also obtained.

Key words: lamin-like proteins, Paramecium

摘要: 本文以DGD包埋去包埋技术对草履虫的核纤层通过透射电镜和免疫荧光、分子杂交等技术进行了观察。结果显示,在核周存在由10nm纤维组成的核纤层;免疫荧光结果表明,在核周呈阳性反应,并在其表皮、口器等部位呈交叉的阳性反应;蛋白分子杂交的反应带在68kD处呈阳性反应。

关键词: 类核纤层蛋白, 草履虫

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