Acta Scientiarum Naturalium Universitatis Pekinensis

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Expression and Properties of the α Domain of Human Metallothionein-3

WANG Hansong,YU Meimin,RU Binggen   

  1. National Laboratory of Protein Engineering, Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing, 100871
  • Received:2000-04-06 Online:2001-07-20 Published:2001-07-20



  1. 北京大学生命科学学院,生物化学与分子生物学系,蛋白质工程国家重点实验室,北京,100871

Abstract: According to the E.coli preference codon, six DNA fragments of α domain of human metallothionein-3(α-MT-3) were synthesized and cloned into the vector pGEX-4T-1. Upon IPTG induction, Glutathione-S-transferase (GST)-α fusion protein was efficiently expressed in E.coli. After digestion by thrombin (combined with CdCl2 or ZnSO4), the desired protein binding Cd2+ or Zn2+ was released, which was confirmed by molecular mass and amino acid composition analysis. Ultraviolet (UV) and Circular Dichroism (CD) spectra were used to test the secondary structure of α-MT-3. Distinct from other α-MT, α-MT-3 showed a negative trough at about 220nm in the CD spectrum, suggesting that an α-helix might exist in its secondary structure.

Key words: metallothionein-3, α-MT-3, expression, structure

摘要: 参考E.coli偏爱密码子,设计并合成人MT-3α结构域(α-MT-3)的DNA片段,克隆到表达载体pGEX-4T-1中。经IPTG诱导,在E.coli中高效表达出谷胱甘肽-S-转移酶(GST)-α融合蛋白。凝血酶酶切融合蛋白(用CdCl2或ZnSO4辅助)后,得到纯化的结合镉或锌的α-MT-3蛋白。质谱分子量检测及氨基酸组成分析证明得到了目的蛋白。紫外吸收光谱及圆二色性光谱研究重组α-MT-3的结构。MT-3的与其余MT的α结构域不同,α-MT-3的圆二色性光谱在220nm左右为负峰,说明α-MT-3可能包含一个α-螺旋的二级结构。

关键词: α-MT-3, 表达, 结构, 金属硫蛋白-3

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